Aspergillus sydowii ISOLATED FROM TWO

Se analizaron 2/1/uestras de lavado bronquial de un paciente h05pitalisado en la Unidad de Penu/l/ologia del Hospital General Otávio de Freitas, Rec!(e, PE, Brasil. El exá/l/en directo en a/l/bas /l/uestras reveló la presencia de:conidios redondos pequeFíos (aislados y agrupados), hialinos. En la segunda /l/uestra se obsel1'aron paralela/l/ente células de levaduras, ovales, oblongas y ge/l/antes. Se obtuvieron en al/Ibas /l/uestras, cultivos puros de A5pergi/lus sydowii y Candida albicll11S.


MA TERIALS AND METHODS
Two samples of bronchial lavage, collected 3 months apart, were processed from a 39 year old, male palient, a native of Recife. hospitalized in the Pneumology Unit ofthe Otavio de Freilas (SANCHO) General Hospi-taL ",ho had bcen diagnosed with diabetes mellitus and puhnOlléll)' tuberculosis with a Iloll-realized resistant Bacillus-aIcohol-acid (BAAR). with negative histopathological neoplasic cells, and positive for filamentous fungi . The two samples of bronchial lavage were obtained and supplied by bronchoscopy and had been sent to the Mycology Department where they were duly processed for direct examination and culture. The time between collection and manipulation ofthe c1inical sampies did not exceed 2 hours.
The direct examination of the bronchial lavage samples was undertaken on their native state (without colouring or c1arifier).
The cultures which arose after being purified were maintained in the above-mentioned mcans of culture without antibiotics and held in a test tube.
For identification and c1assification Raper & Fennel (1965) were consultcd for the Aspergillus strains as well Kreger-van Rij (1984) and Barnett et al. (1990) for the yeast strain.

RESULTS
The direct examination of the 2 bronchial lavage samples revealed the presence of small, round hialine conidia, isolated and grouped; in the second sample, apart from the spores already mentioned, the presence of oval and oblong yeast cells with simple budding was noted.
The Aspergillus pure cultures obtained from the 2 samples of bronchial lavage in Sabouraud agar +YE+chlorophenicoL both at RT and 37° e, were idcntified in Czapec and Malt agar as A. sydowii . After being purified, the Candida cultures obtained from the second sample ofbronchiallavage were identified as C. alhicans.
The 2 samples of A. sydowii (3631 and 3632) and C. alhicans (3620) are deposited in the URM-Mycotheca of the Mycology Department, Centre of Biological Sciences, Federal University ofPernambuco, Recife, PE, Brazil.

DISCUSSION
In the literature the association between pulmonary aspergilosis and pulmonal)' tuberculosis was reported by Tomlinson &Steven (1987), andKumar et al. (1992) and with diabetes mellitus by Karen et al. (1988).
Through c1inical, histopathological and serological examinations, as well as mycological examination of the sputum and bronchial secretion, pulmonary aspergilosis cases were diagnosed which have the species A. fumigatus, A. niger andA.flavus ( Tomlinson & Steven , 1987: Kumar et al. , 1992 as etiological agents. Bandele et al. (1993), howeverconsider the presence ofA.jumigatus,A. niger and Aspergillus sp. in the sputum of patients as pulmonary tuberculosis as an infection and not as pulmonary aspergillosis. The results obtained in this work showed the presence of A. sJ'dowii (Section Versicolore,~) (W. Gams et al. 1985) in the bronquiallavage samples.
There is no reference in the literature about tile occurrence of A. sydowii in respiratory systems samples Thus we can conclude that A. sJ'dowii is being mentioned for the first time as a fllIlgi present in bronquial lavage samples detected from direct and culture examination.
It \Vas not possible to get further c1inica! data on this case so we focused mercly on this interesting isolation witilout considering possible derived pulmonary aspergillosis.