Dichotomaria marginata (Rhodophyta) as a bioindicator for marine pollution: An overview about its metabolites and adsorbed pollutants

Macroalgae are considered bioindicators for marine pollution, because they have the ability to quickly react to changes in their environment. In consequence, macroalgae populations fluctuate, according to species characteristics and adaptive strategies. Their cell wall polysaccharides contain sulfate groups that are capable of retaining and accumulating heavy metals. In addition to traditional contaminants, emerging pollutants are being recognized in aquatic environments. Herein, emerging pollutants have been identified after being desorbed from the macroalga Dichotomaria marginata, collected from Fortaleza Beach, Ubatuba SP, Brazil. Based on that algal polysaccharide networks have the potential of forming hydrogen bonds with polar compounds, it was hypothesized that these pollutants would be bound to sugar polymers. Compounds present in the D. marginata samples were identified using both gas and liquid chromatography/mass spectrometry (GC/MS and HPLC/MS), assisted by computational methods. It was possible to unequivocally identify 22 emerging contaminants with GC/MS, and 16 substances with HPLC/MS.


Introduction
Over a century ago it was first proposed that there is a connection between the presence, absence or abundance of algal species and the characteristics of the marine environment (Kolkwitz & Marsson 1908). In fact, many species of algae have adapted to eutrophic conditions by undergoing morphological, physiological and/or ecological changes, which promote the growth of the population (Omar 2010), and the disappearance of others coincides with habitat eutrophication (Sousa & Cocentino 2004, Holt & Miller 2010, Omar 2010.
Previous studies have demonstrated that algae have the ability to incorporate heavy metals and nutrients (Omar 2010, Rajfur 2014, Rajfur & Kłos 2014. This characteristic is attributed to the high adsorption power of the thallus, whose cell walls are made up of polysaccharides, proteins, and lipids. The polysaccharide composition of the cell walls and the of the intercellular matrix can differ in different groups of red algae, but related to storage carbohydrates, there are basically two types of them (floridosides and digeneaside).
In the marine environment, all organisms are in constant contact with dissolved or suspended substances. For example, in addition to common salts, bases, and organic or inorganic acids, marine organisms are also in contact with halogens, alkali metals, oxygen, nitrogen, silicon, trace elements, organic compounds (produced by aquatic organisms themselves) and pollutants (Bryan 1979, Millero et al. 2008).
Among these pollutants are emerging contaminants, which are a group of substances used extensively in everyday life. These contaminants are not currently included in routine water monitoring programs, norms or legislation for environmental control (Calvo-Flores et al. 2018). This is primarily because the existing toxicity data are insufficient for establishing the respective reference doses. As a consequence, these unregulated pollutants pose a serious challenge to water quality regulators (Barceló 2003, Farré et al. 2008, Sousa et al. 2018. Furthermore, these agents have been detected in numerous aquatic organisms (Mearns et al. 2015).
These polysaccharides contain a large amount of hydroxyl groups, which endows these carbohydrates with a hydrophilic character and the ability to form hydrogen bonds with environmental molecules. Hydrogen bonds occur when the hydrogen atom bound covalently to an electronegative atom (hydrogen donor group) electrostatically interacts with electronegative atoms (hydrogen receptor) forming non-covalent bonds. In the hydrogen donor group, usually the electronegative atom is either oxygen, nitrogen or sulfur. Hydrogen receptors are typically O, N, S and also phenols (Schaeffer 2008).
The lipids contain carboxylate, sulfate and phosphate anionic groups, which function as metal-binding sites, as well as positively charged groups. Additionally, environmental pH and the presence of competing ions can impact the adsorption process (Gadd 2009, Chopra & Pathak 2010. Algae also adsorb pollutants through the use of phytochelatins, which are peptides that are capable of chelating heavy metals (Inouhe 2005). With regards to macroalgae, compounds such as: surfactants, phthalates and polychlorinated biphenyls have been isolated from their extracts (Mackintosh et al. 2004, Gressler et al. 2012, Osman et al. 2013, Shobier et al. 2016, Stranska-Zachariasova et al. 2017. These three groups of compounds have antifungal activity (Uyanik et al. 2009, Memić et al. 2011, Lotfy et al. 2018, Paluch et al. 2018, Fait et al. 2019). In the late 1970s, phthalates were identified as algal components (Noguchi et al. 1979), and the bioconcentration, as well as the trophic magnification in the aquatic food web have been evaluated (Mackintosh et al. 2004).
In studies with algae, gas chromatography/mass spectrometry (GC/MS) has been utilized for the detection of phthalates, adipates and surfactants, due to the inherent thermal stability, volatility and of low polarity of these compounds (Mackintosh et al. 2004, Gressler et al. 2012, Osman et al. 2013, Shobier et al. 2016, Stranska-Zachariasova et al. 2017). On the other hand, high performance liquid chromatography coupled with mass spectrometry (HPLC/MS) has been successfully employed in research on non-volatile, thermally unstable and more polar contaminants (Goulitquer et al. 2012).
Both analytical platforms (GC/MS and HPLC/MS) become more valuable when assisted by computer tools such as the AMDIS (Automated Mass Spectrometry Deconvolution and Identification System) algorithm, which can separate co-eluting components (deconvolution), provide accurate molecular weights and generate both qualitative and quantitative data for each substance (Du & Zeisel 2013).
The search for biological activities in algae extractswhether they are polar or nonpolar -is the starting point for the discovery and isolation of substances with interesting biological actions. Regarding antifungal activity, it was detected in the hexane extracts of Dictyota dichotoma, Dictyota dichotoma var. implexa and Dilophus spiralis (Moreau et al. 1988) and of Laurencia species (Stein et al. 2011)  Thus, the present study was designed to identify metabolites from the algae and potential emerging pollutants, desorbed from the thallus of the benthic marine macroalgae Dichotomaria marginata (J. Ellis & Solander) Lamark, 1816, which may have antifungal activity, using a combination of GC/MS and HPLC/MS, followed by spectral deconvolution. The results will demonstrate the utility of D. marginata as a bioindicator for emerging contaminants and the interference of some of these contaminants in biological activity assays of crude extracts.

Sample collection and extraction
Dichotomaria marginata, previously named as Galaxaura marginata, is a calcareous alga that belongs to the Rhodophyta (Johnson et al. 2014). Samples of this species were manually collected from Fortaleza Beach, in Ubatuba, São Paulo, Brazil (23º50'15"S / 40º17'40"W), during low tides. A voucher specimen (SP 428.540) was deposited at the Maria Eneyda P. Kauffman Fidalgo Herbarium, Instituto de Botânica, São Paulo.
Following collection, samples were washed, frozen and transported inside thermal box with ice to the laboratory. At the laboratory, they were shade dried at room temperature (20-25 °C), and ground into fine particles (Carvalho et al. 2003). The powdered biomass (419.63 g) was extracted with hexane (5× 3.45 L) until exhaustion; the pooled extracts were filtered through filter paper (Whatman® No. 5), the solvent was removed under reduced pressure and the final product was weighed (Bhagavathy et al. 2011).

Re-fractionation of pooled fractions from C-I
Combined fractions from C-I (Fractions 4-9) (53.4 mg) were refractionated on a silica gel column (designated as C-II), using DCM/MeOH 99.5:0.5 v/v as the mobile phase, which yielded 55, 1 mL, fractions. After PC analysis, similar fractions were combined. The combined fractions from both columns (C-I and C-II) were then analyzed by GC/ MS and HPLC/MS.
Vol. 55, N°2, 2020 Revista de Biología Marina y Oceanografía Gas chromatography and mass spectrometry GC/MS analyses of all samples were performed using a mass spectrometer connected to a Shimadzu GCMS-QP2010 plus gas chromatograph (Kyoto, Japan), equipped with a HP-5MS column (5%-phenylmethylpolysiloxane, 30 m × 0.25 mm id, 0.25 μm film thickness). Helium gas was used as the carrier, at a flow rate of 1.0 mL/min. The injector temperature was 250 ºC and the oven temperature was initially set at 60 ºC and increased at 3 ºC per min, until reaching 260 ºC, at which time this temperature was held for 40 min.
The mass spectrometer was operated in full scan mode from 40 to 1000 m/z, at an interface temperature of 240 ºC, and the samples were ionized with an electron beam of 70 eV.
Identification of chemical constituents was performed by comparing the mass spectra with data from the NIST/ EPA/NIH Mass Spectral Library (Version 2.0).
The linear retention indices were calculated according to the Kovats method (KI), using a mixture of C6-C28 n-alkanes as external references (Sigma-Aldrich, St. Louis, MO, USA).
Only compounds with retention times and fragmentation patterns that matched reference compounds or spectral data available in literature were considered reliable, and thus included in the text.

HPLC-ESI-EM
These analyses were carried out using a Bruker HPLC instrument (MicroTOFII) coupled to a diode-array detector and a mass spectrometer with electrospray ionization (ESI-MS). The mass spectrometer was operated in the positive ion mode and provided mass spectra data from m/z 50 to 1000.

Thin-layer chromatography/bioautography for detection of antifungal activity
The assay was conducted using the microorganism Cladosporium cladosporioides Fresen (SPC 140), which have been maintained at the Instituto de Botânica, São Paulo, Brazil. This fungus was cultured on potato dextrose agar (Difco) for 12 days until sporulation. The spore suspension was then extracted in a solution containing glucose and salt (Homans & Fuchs 1970, Rahalison et al. 1994) to a final concentration of 108 spores mL −1 and used for qualitative assessment of the antifungal activity of the crude seaweed extract and fractions.
The test was carried out by applying 10 μL of a solution containing 100 μg of crude extract or fractions on silica gel GF 254 TLC plates (Merck, Germany) in DCM/MeOH (98.5:1.5 v/v), which were developed in tanks lined with solvent-saturated Whatman No. 3M. After thorough air drying, the chromatograms were sprayed with fungal spore suspensions and incubated for 72 h at 28 °C. Antifungal compounds on the developed plates appeared as clear zones against the dark backgrounds of the TLC plates. Nystatin was used as positive controls.

Results
In Figure 2A, the inhibitory activity exerted by a D. marginata hexane extract against the fungi Cladosporium cladosporioides is shown. Likewise, the Figure 2B Table 1; among them, there are the diterpene Phytol, which is a product of Chlorophyll degradation (Olofsson et al. 2014) and Demosterol, a steroid commonly found in red alga (Sharanagat et al. 2019). Substances that can be used to toiletries and personal care products, which were also herein identified, are in Table 2. They include myristic, oleic and palmitoleic acids, compounds produced by algae but that are also synthesized on an industrial scale to supply cosmetic companies (Gressler et al. 2010, Zielinska & Nowak 2014.

Table 1. Substances synthesized by algae and identified in D. marginata by gas chromatography/mass spectrometry (GC/MS) /
The emerging pollutants detected by GC/MS are shown in Table 3. In total, 23 compounds were identified, with industrial contaminants being the most prevalent, followed by personal care products (Octinoxate). Pharmaceuticals (Pregnenolone methyl ether), household cleaning products (Cyclohexasiloxane), food additives (Ciclotene), endocrine disruptors (Nonylphenol) and plasticizers (Diisobutyl phthalate) were also identified.
Vol. 55, N°2, 2020 Revista de Biología Marina y Oceanografía Table 2. Substances synthesized by algae and produced for various industrial uses, identified in D. marginata by gas chromatography/mass spectrometry (GC/MS) / Sustancias sintetizadas por algas y producidas para varios usos industriales, identificadas en D. marginata por cromatografía de gases/espectrometría de masas (GC/MS) Dichotomaria marginata bioindicator of pollution Table 3. Emergent pollutants identified in D. marginata by gas chromatography/mass spectrometry (GC/MS) / Contaminantes emergentes identificados en D. marginata por cromatografía de gases/espectrometría de masas (GC/MS)  The sixteen pollutants which were identified by HPLC/ MS technique compounds are gathered in Table 4. Seven of these compounds are used for personal care, four as food additives, and two are pharmaceuticals. In fact, the drugs dipyrone and ethinylestradiol were only detected by HPLC/MS. Figure 3 shows the structural formulas of some of these pollutants (Kim et al. 2019).

Discussion
Hexane extracts of D. marginata presented, in addition to its typical components, compounds with different origins and potential applications, indicating that the species is able to retain them in its thallus.
The main components of the hexane extracts are usually compounds of low molecular weight and low polarity, such as some hydrocarbons, fatty acids, terpenes and aromatics compounds. Antibiotic activities of any kind which were assigned to these extracts (essential oils) oftentimes are due to terpenes and aromatic compounds (Nazzaro et al. 2017).
Among the primary metabolites herein identified (Table  1)  Special mention should be made of fatty acids C 12 , C 14 and C 16 (Table 2), since they are valuable components of the human diet, precursors of the eicosanoid biosynthesis and bioregulators of several cellular processes (Gressler et al. 2010). Additionally, these compounds are considered to be a useful tool in chemotaxonomy, since they help to distinguish classes, families and genera of macroalgae (Khotimchenko 2005).
Among the compounds listed in Table 3, which are all pollutants, phthalates deserve special attention since they are very often isolated from macroalgae and sometimes mistakenly regarded as the natural components of these organisms (Chan et al. 2004, Gressler et al. 2012, Avio et al. 2016). Phathalates are not only antifungal compounds but also they may have toxic effects on humans and animals (Rowdhwal & Chen 2018). Still in Table 3, various other substances which have adverse effects on living beings are listed (references cited in Table 3).
Apart from four variants of phthalates, the pollutants nonyphenol (Karley et al. 1997), and cyclohexasiloxane (Moustafa et al. 2013) have antifungal activity as well. The fungitoxic action of these compounds could justify much of the observed fungal inhibition, since only one algal metabolite showed this activity.
The adsorption of these pollutants relied on the ability of the structural polysaccharides of the macroalgae to sequester and accumulate substances, with which these compounds can electrostatically bond.
On the other hand, the molecules of these substances must have oxygen atoms (hydrogen receptors) which are the ones that make up carbonyl (from acids, esters and ketones) and phenolic hydroxyl groups as well, nitrogen atoms which make up amines and amides and sulfur atoms, for sulfate groups (Paoloni et al. 1975, Hay et al. 2004, Schaeffer 2008, Ouellette & Rawn 2015. The molecules of the sixteen pollutants herein identified have groups which can act as hydrogen receptors, by bonding with the numerous hydroxyl groups of sugar polymers, forming hydrogen bonds. The pollutants octinoxate, diisobutyl phthalate, homomenthyl salicylate, methyl glycol phthalate, pregnenolone 3-methyl ether, erucylamide, n-butyl-9-octadecenamide, oxybenzone, tributyl acetyl citrate, dipyrone, and 2-hydroxy-3-methyl-2cyclopenten-1-one have a caboxyl group. Ethinylestradiol, oxybenzone, nonylphenol, and homomenthyl salicylate are phenolic substances; erucylamide and n-butyl-9octadecenamide are amides and dipyrone is a sulfated substance (Fig. 3).
While the GC/MS method reliably identified emerging pollutants desorbed from the thallus of D. marginata, the HPLC/MS procedure, assisted by spectral deconvolution, proved to be a valuable tool for identifying non-metallic, polar, non-volatile and thermally unstable environmental contaminants. These types of compounds are often present in minute quantities in samples with a profuse mixture of compounds (Magi & Di Carro 2016, Martín et al. 2017).
In conclusion, the significant number of pollutants detected by both chromatographic methods (GC and HPLC) indicate that macroalgae have the ability to retain a variety of substances dissolved or suspended in the aquatic environment. Due to the fact that some of the identified compounds were from pharmaceuticals residues and industrial debris, the present study has demonstrated that the red alga Dichotomaria marginata could be a reliable bioindicator for water pollution. Early detection and subsequent remediation will minimize the harmful effects associated with these pollutants, and benefit the human population, in general.