IonOptix Platform and cardiomyocyte calcium handling
DOI:
https://doi.org/10.22370/syc.2.1.2026.5684.Keywords:
Excitation-Contraction Coupling, Cardiomyocyte Calcium Handling, IonOptix Platform, Diabetic Cardiomyopathy, Ischemia-Reperfusion InjuryAbstract
Excitation-contraction coupling is the basic calcium-mediated physiological regulation of cardiac contraction. It is critical for cardiac function, and its dysregulation is a key mechanism in heart disease pathology. This view describes the essential role of calcium management in cardiomyocytes and advances the IonOptix platform as an excellent ex vivo mechanism instrument of the above view in the mechanistic study. IonOptix allows for simultaneous, real-time assessment of intracellular Ca²+ transients and sarcomere shortening in single cardiomyocytes, allowing a complete functional reading of systolic and diastolic ratios. This reductionist approach is a powerful tool for identifying primary cellular pathophysiology, characterizing disease model phenotypes, and conducting drug screening. Here, we discuss its implementation in the Cardiovascular Physiology Laboratory at the Universidad de Valparaíso. We discuss the application to the Cardiovascular Physiology Laboratory of the University of Valparaíso, focused specifically on experimental models of diabetic cardiomyopathy and ischemia-reperfusion injury, as well as research collaboration that advances the understanding of new compounds with activity in the cardiovascular system.
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