Keywords
angiogénesis
osteogénesis
plasma rico en fibrina
aloinjerto óseo
inmunofluorescencia
How to Cite
Abstract
Introduction: Tooth loss leads to a series of morphological changes associated with alveolar bone resorption, which represents a limitation in rehabilitation treatments with dental implants. Various techniques and biomaterials have been developed to promote bone regeneration in this context. Angiogenesis plays an essential role in osteogenesis by facilitating the supply of nutrients and oxygen necessary for the formation of new bone tissue. This study aims to evaluate the expression of angiogenic (vWF, CD31) and osteogenic (OCN) markers in native and regenerated alveolar bone after maxillary sinus elevation with freeze-dried particulate allograft and fibrin-rich plasma (PRF). Materials and
Methods: Bone biopsies from three patients, obtained between 6 and 8 months after the regeneration procedure, were analyzed. The samples were divided into caudal (native bone) and cranial (regenerated bone) areas and processed for histological analysis by optical and confocal microscopy, with immunostaining for vWF, CD31, and OCN.
Results: Angiogenic markers were identified in both zones, with higher microvascular density in areas adjacent to graft particles. Osteocalcin showed evident expression in the regenerated bone, with the presence of osteoid tissue and reticular bone in formation, indicating osteogenic activity.
Conclusions: This pilot case series demonstrates the involvement of angiogenesis and osteogenesis in alveolar bone regeneration following sinus elevation with PRF and allograft. The findings support the biological viability of this combination and justify further studies with larger sample sizes and quantitative analysis.
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