Caracterización de la actividad biológica del extracto crudo proteico de la estrella de mar Luidia magnifica
DOI:
https://doi.org/10.22370/rbmo.2024.59.2.4807Palabras clave:
Equinodermos, extracto proteico, bioactividad, hemólisis, citotoxicidadResumen
Los equinodermos han sido reconocidos como una fuente rica de moléculas activas, con aplicaciones farmacológicas prometedoras en el campo de las investigaciones biomédicas. El objetivo del presente estudio fue evaluar la actividad biológica del extracto de naturaleza proteica de la estrella de mar Luidia magnifica mediante bioensayos citolíticos y citotóxicos. A partir de los brazos de la estrella de mar se obtuvo el extracto crudo, que demostró actividad antimicrobiana contra Escherichia coli y Candida albicans (CMI= 1,04 mg mL-1 y 0,52 mg mL-1, respectivamente), actividad hemolítica dosis dependiente (94,8% y EC50= 2,929 mg mL-1) sobre eritrocitos humanos, así como actividad citotóxica frente a la línea celular de glioma de rata C6, produciendo una inhibición celular de 52% y 32% de viabilidad celular, a una concentración de 20 mg mL-1 con los métodos de tinción rojo neutro y cristal violeta, respectivamente. Los resultados sugieren que la estrella de mar L. magnifica representa una fuente potencial de sustancias biológicamente activas que pudieran ser precursores de compuestos para el tratamiento de enfermedades.
Citas
Anbukkarasu S, B Subramanian, N Elayaperumal & M Masilamani. 2014. Antimicrobial properties of partially purified extract of starfish Luidia maculata. International Journal of Natural Products Research 4(4): 100-104.
Andriani Y, NHM Lazim, A Asari, F Mohamad, T Sifzizul, TST Muhammad, N Ismail, M Taib, H Amir, A Ahmad & H Mohamad. 2018. Evaluation of selected echinoderms from peninsular Malaysia for cytotoxicity against HepG2 cells, antioxidant and antibacterial activities, and their metabolites profiling. Journal of Applied Pharmaceutical Science 8(10): 32-38.
Arizza V & D Schillaci. 2016. Echinoderm antimicrobial peptides: The ancient arms of the deuterostome innate immune system. In: Ballarin L & M Cammarata (eds). Lessons in immunity: from single-cell organisms to mammals, pp. 159-176. Academic Press, New York.
Atil H & Y Unver. 2001. Multiple comparisons. Journal of Biological Sciences 1(8): 723-727.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1/2): 248-254.
Canicatti C. 1989. Evolution of the lytic system in echinoderms. II. Naturally occurring hemolytic activity in Marthasterias glacialis (Asteroidea) coelomic fluid. Comparative Biochemistry and Physiology 93(3): 587-591.
Canicatti C & D Ciulla. 1988. Studies on Holothuria polii (Echinodermata) coelomocyte lysate II. Isolation of coelomocyte hemolysins. Developmental & Comparative Immunology 12(1): 55-63.
Canicatti C & P Roch. 1989. Studies on Holothuria polii (Echinodermata) antibacterial proteins. I. Evidence for and activity of a coelomocyte lysozyme. Experientia 45: 756-759.
Chiba K, K Kawakami & K Tohyama. 1998. Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells. Toxicology in Vitro 12: 251-258.
Coté RJ. 1999. Sterilization and filtration. Current Protocols in Cell Biology 1(1): 1-21.
Dalgaard P, T Ross, L Kamperman, K Neumeyer & TA McMeekin. 1994. Estimation of bacterial growth rates from turbidimetric and viable count data. International Journal of Food Microbiology 23(3-4): 391-404.
Datta D, SN Talapatra & S Swarnakar. 2015. Bioactive compounds from marine invertebrates for potential medicines - An overview. International Letters of Natural Sciences 7: 42-61.
Dennis EA. 1983. Phospholipases. In: Boyer PD (ed). The enzymes. Lipid enzymology 16: 307-353. Academic Press, New York.
Folmer F, M Jaspars, M Schumacher, M Dicato & M Diederich. 2010. Marine natural products targeting phospholipases A2. Biochemical Pharmacology 80(12): 1793-1800.
Franco O, G Patiño & A Ortiz. 2015. Actividad antibacteriana y antifúngica de la estrella de mar Oreaster reticulatus (Valvatida: Oreasteridae) y de los erizos de mar Mellita quinquiesperforata (Clypeasteroida: Mellitidae) y Diadema antillarum (Diadematoida: Diadematidae) del Caribe Colombiano. Revista de Biología Tropical 63(2): 329-337.
Galván-Villa CM, FA Solís-Marín, K Lopez, J Cobb, L Díaz-Pérez, CR Rezende-Ventura, N Slivak, CT Ford & DA Janies. 2024. Occurrence of the Indo-West Pacific starfish Luidia magnifica (Echinodermata: Asteroidea) in the Mexican Pacific and a possible introduction to the Caribbean region. Marine Biodiversity 54(1).
Gomes AR, AC Freitas, TAP Rocha-Santos & AC Duarte. 2014. Bioactive compounds derived from echinoderms. Royal Society of Chemistry 4(56): 29365-29382.
Gomes AR, AC Freitas, AC Duarte & TAP Rocha-Santos. 2016. Echinoderms: a review of bioactive compounds with potential health effects. In: Rahman AU (ed). Studies in Natural Products Chemistry 49: 1-54. Elsevier, Amsterdam.
Gowda NM, U Goswami & MI Khan. 2008. Purification and characterization of a T-antigen specific lectin from the coelomic fluid of a marine invertebrate, sea cucumber (Holothuria scabra). Fish & Shellfish Immunology 24(4): 450-458.
Gross PS, WZ Al-Sharif, LA Clow & LC Smith. 1999. Echinoderm immunity and the evolution of the complement system. Developmental and Comparative Immunology 23(4-5): 429-442.
Habermann E & KL Hardt. 1972. A sensitive and specific plate test for the quantitation of phospholipases. Analytical Biochemistry 50(1): 163-173.
Hashimoto Y. 1979. Marine toxins and other bioactive marine metabolites, 369 pp. Japan Scientific Societies Press, Tokyo.
Hatakeyama T, H Kohzaki, H Nagatomo & N Yamasaki. 1994. Purification and characterization of four Ca2þ-dependent lectins from the marine invertebrate, Cucumaria echinata. Journal of Biochemistry 116: 209-214.
Haug T, AK Kjuul, OB Styrvold, E Sandsdalen, ØM Olsen & K Stensv. 2002. Antibacterial activity in Strongylocentrotus droebachiensis (Echinoidea), Cucumaria frondosa (Holothuroidea), and Asterias rubens (Asteroidea). Journal of Invertebrate Pathology 81(2): 94-102.
Ibrahim RF, N Widhyastuti, IKE Savitri, M Sahlan & A Wijanarko. 2013. Antibacterial investigated of phospholipase A2 from the spines venom of crown of thorns starfish Acanthaster planci. International Journal of Pharma and Bio Sciences 4(2): B1-B5.
Iorizzi M, S DeMarino & F Zollo. 2001. Steroidal oligoglycosides from the asteroidea. Current Organic Chemistry 5: 951-973.
Jha RK & X Zi-Rong. 2004. Biomedical compounds from marine organisms. Marine Drugs 2(3): 123-146.
Jolles J & P Jolles. 1975. The lysozyme from Asterias rubens. European Journal of Biochemistry 54(1): 19-23.
Kamiya H, K Muramoto, R Goto & M Sakai. 1992. Lectins in the hemolymph of a starfish, Asterina pectinifera: purification and characterization. Developmental & Comparative Immunology 16(2-3): 243-250.
Kelly MS. 2005. Echinoderms: their culture and bioactive compounds. In: Matranga V (ed). Echinodermata. Progress in molecular and subcellular biology 39: 139-165. Springer, Berlin.
Kim CH, HJ Go, HY Oh, JB Park, TK Lee, JK Seo, MR Elphick & GN Park. 2018. Identification of a novel antimicrobial peptide from the sea star Patiria pectinifera. Developmental and Comparative Immunology 86: 203-213.
Kishimura H. 2012. Enzymatic properties of starfish phospholipase A2 and its application. In: Kim SK (ed). Marine medicinal foods. Implications and applications - Animals and microbes. Advances in Food and Nutrition Research 65: 437-456. Elsevier, Waltham.
Kishimura H & K Hayashi. 2005. Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis. Food Chemistry 92(3): 407-411.
Koyama N, H Kishimura & K Hayashi. 2004. Partial purification and characteristics of phospholipase A2 from pyloric ceca of starfish Plazaster borealis. Bulletin of Fisheries Sciences, Hokkaido University 55(1): 11-16.
Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259): 680-685.
Leonard LA, JD Strandberg & JA Winkelstein. 1990. Complement-like activity in the sea star, Asterias forbesi. Developmental & Comparative Immunology 14(1): 19-30.
Li C, T Haug, OB Styrvold, TØ Jørgensen & K Stensvåg. 2008. Strongylocins, novel antimicrobial peptides from the green sea urchin, Strongylocentrotus droebachiensis. Developmental and Comparative Immunology 32(12): 1430-1440.
Li C, T Haug & K Stensvåg. 2010. Antimicrobial peptides in echinoderms. Invertebrate Survival Journal 7(7): 132-140.
Li C, HM Blencke, T Haug & K Stensvåg. 2015. Antimicrobial peptides in echinoderm host defense. Developmental and Comparative Immunology 49(1): 190-197.
Macedo MWFS, NBD Cunha, JA Carneiro, RAD Costa, SAD Alencar, MH Cardoso, OL Franco & SC Dias. 2021. Marine organisms as a rich source of biologically active peptides. Frontiers in Marine Science 8: 667764.
Matranga V. 1996. Molecular aspects of immune reactions in Echinodermata. In: Rinkevich B & WEG Müller (eds). Invertebrate Immunity. Progress in molecular and subcellular biology 15: 235-247. Springer, Berlin.
Mercado L, P Schmitt, SH Marshall & G Arenas. 2005. Gill tissues of the mussel Mytilus edulis chilensis: A new source for antimicrobial peptides. Electronic Journal of Biotechnology 8(3): 284-290.
Moguel-Salazar F, E Ortiz-Vázquez, R Rodríguez-Canul & L Olivera-Castillo. 2013. Antimicrobial activity of aqueous extracts of sea cucumber (Isostichopus badionotus) from the coast of Yucatan, Mexico. African Journal of Microbiology Research 7(28): 3621-3626.
Montaser R & H Luesch. 2011. Marine natural products: a new wave of drugs? Future Medicinal Chemistry 3(12): 1475-1489.
Moura RDM, KS Aragão, AA de Melo, RF Carneiro, CB Osório, PB Luz, AFS de Queiroz, EA dos Santos, NMH de Alencar & BS Cavada. 2013. Holothuria grisea agglutinin (HGA): the first invertebrate lectin with anti‐inflammatory effects. Fundamental & Clinical Pharmacology 27(6): 656-668.
Mutee AF, SM Salhimi, FC Ghazali, FM Al-Hassan, CP Lim, K Ibrahim & MZ Asmawi. 2012. Apoptosis induced in human breast cancer cell line by Acanthaster planci starfish extract compared to tamoxifen. African Journal of Pharmacy and Pharmacology 6(3): 129-134.
Okabe H & A Noma. 1973. Studies on the phospholipase A2 in pyloric caeca of the starfish Asterina pectinifera. The Tohoku Journal of Experimental Medicine 110(3): 263-271.
Ong P, T Ohtake, C Brandt, I Strickland, M Boguniewicz, T Ganz & DY Leung. 2002. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. New England Journal of Medicine 347(15): 1151-1160.
Osada M, T Ito, T Matsutani & K Mori. 1993. Partial purification and characterization of hemolysin from the coelomic fluid of Strongylocentrotus nudus. Comparative Biochemistry and Physiology B 105(1): 43-49.
Parajuli K, N Fahim, S Mumu, R Palu & A Mustafa. 2023. Antibacterial potential of Luidia clathrata (sea star) tissue extracts against selected pathogenic bacteria. PLoS ONE 18(3): e0281889.
Price MF, AND Wilkinson & LO Gentry. 1982. Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia-Journal of Medical and Veterinary Mycology 20(1): 7-14.
Ramírez-Gómez FJ & JE García-Arrarás. 2010. Echinoderm immunity. Invertebrate Survival Journal 7(2): 211-220.
Repetto G, A Peso & JL Zurita. 2008. Neutral red uptake assay for the estimation of cell viability/ cytotoxicity. Nature Protocols 3(7): 1125-1131.
Ridzwan BH, MA Kaswandi, Y Azman & M Fuad. 1995. Screening for antibacterial agents in three species of sea cucumbers from coastal areas of Sabah. General Pharmacology 26(7): 1539-1543.
Rinehart KL, PD Shaw, LS Shield, JB Gloer, GC Harbour, MES Koker, D Samain, RE Schwartz, AA Tymiak, DL Weller, GT Carter, MHG Munro, RG Hughes, HE Renis, EB Swynenberg, DA Stringfellow, JJ Vavra, JH Coats, GE Zurenko, SL Kuentzel, LH Li, GJ Bakus, RC Brusca, LL Craft, DN Young & JL Connor. 1981. Marine natural products as sources of antiviral, antimicrobial and antineoplastic agents. Pure & Applied Chemistry 53(4): 795-817.
Rottini G, A Dobrina, O Forgiarini, E Nardon, GA Amirante & P Patriarca. 1990. Identification and partial characterization of a cytolytic toxin produced by Gardnerella vaginalis. Infection and Immunity 58(11): 3751-3758.
Ru R, Y Guo, J Mao, Z Yu, W Huang, X Cao, H Hu, M Meng & L Yua. 2022. Cancer cell inhibiting sea cucumber (Holothuria leucospilota) protein as a novel anti-cancer drug. Nutrients 14(4): 786.
Sallay I, S Moriwaki, O Nakamura, S Yasuda, M Kimur, N Yamasak, K Itoh & H Ohba. 2000. Interaction of the hemolytic lectin, CEL-III, with cultured human leukemic cell lines. Journal of Hematotherapy & Stem Cell Research 9(6): 877-883.
Schillaci D, MG Cusimano, A Spinello, G Barone, D Russo, M Vitale, D Parrinello & V Arizza. 2014. Paracentrin 1, a synthetic antimicrobial peptide from the sea-urchin Paracentrotus lividus, interferes with staphylococcal and Pseudomonas aeruginosa biofilm formation. AmB Express 4(78): 1-9.
Schumacher MJ, JO Schmidt, NB Egen, KA Dillon & BA Tucson. 1992. Biochemical variability of venoms from individual European and Africanized honeybees (Apis mellifera). Journal of Allergy and Clinical Immunology 90(1): 59-65.
Service M & AC Wardlaw. 1984. Echinochrome-A as a bactericidal substance in the coelomic fluid of Echinus esculentus (L.). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 79(2): 161-165.
Shimada S. 1968. Antifungal steroid glycoside from sea cucumber. Science 163(3874): 1462-1462.
Shiomi KA, A Kazama, K Shimakura & Y Nagashima. 1998. Purification and properties of phospholipases A2 from the crown-of-thorns starfish (Acanthaster planci) venom. Toxicon 36(4): 589-599.
Simmons TL, E Andrianasolo, K Mcphail, P Flatt & WH Gerwick. 2005. Marine natural products as anticancer drugs. Molecular Cancer Therapeutics 4(2): 333-343.
Smith LC, J Ghosh, KM Buckley, LA Clow, NM Dheilly, T Haug, JH Henson, C Li, CM Lun, AJ Majeske, V Matranga, SV Nair, JP Rast, DA Raftos, M Roth, S Sacchi, CS Schrankel & K Stensvåg. 2010. Echinoderm immunity. In: Söderhäll K (ed). Invertebrate immunity 708: 260-301. Springer, Boston.
Stabili L, P Pagliara & P Rocm. 1996. Antibacterial activity in the coelomocytes of the sea urchin Paracentrotus lividus. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 113(3): 639-644.
Stabili L, MI Acquaviva, RA Cavallo, C Gerardi, M Narracci & P Pagliara. 2018. Screening of three echinoderm species as new opportunity for drug discovery: their bioactivities and antimicrobial properties. Evidence-Based Complementary and Alternative Medicine 2018(1): 1-8.
von Schwarzenberg K & AM Vollmar. 2013. Targeting apoptosis pathways by natural compounds in cancer: marine compounds as lead structures and chemical tools for cancer therapy. Cancer Letters 332(2): 295-303.
Wijanarko A, K Lischer, H Hermansyah, DK Pratami & M Sahlan. 2018. Antiviral activity of Acanthaster planci phospholipase A2 against human immunodeficiency virus. Veterinary World 11(6): 824-829.
Xu T, B Yang, X Lin, X Zhou, X Yang & Y Liu. 2011. Chemical constituents and bioactivities of starfish. Chemistry & Biodiversity 8(5): 740-791.
Zar JH. 2010. Biostatistical analysis, 944 pp. Prentice-Hall/Pearson, Upper Saddle River.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2024 María Ivonne García-Quezada, Cristian Moisés Galván-Villa, Alfonso Enrique Islas-Rodríguez, Laura Guadalupe Medina-Ceja, Judith Sánchez-Rodríguez
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial 4.0.
• Los autores que publican en la RBMO transfieren sus derechos de publicación a la Universidad de Valparaíso, conservando los derechos de propiedad intelectual para difundir ampliamente el artículo y la revista en cualquier formato.
• La RBMO autoriza el uso de figuras, tablas y extractos breves de su colección de manuscritos, en trabajos científicos y educacionales, siempre que se incluya la fuente de información.
